Biotechnology and Bioprocess Engineering 2024; 29(6): 1071-1080  
Nano-sized lysate of Lactiplantibacillus plantarum isolated from green tea leaves as a potential skin care ingredient
Kilsun Myoung 1,2 · Eun-Jeong Choi 2 · Sehyun Kim 2 · Jeong Ah Hwang 2 · Ji Young Lee 2 · Hyoung-June Kim 2 · Jae Sung Hwang 1
1Department of Genetic and Biotechnology, Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, Korea
2AMOREPACIFIC Research and Innovation Center, Yongin 17074, Korea
Correspondence to: ✉ Hyoung-June Kim
leojune@amorepacifi c.com

✉ Jae Sung Hwang
jshwang@khu.ac.kr
Received: March 13, 2024; Revised: June 26, 2024; Accepted: July 11, 2024; Published online: August 21, 2024.
© The Korean Society for Biotechnology and Bioengineering. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Fermentation products and lysates of lactic acid bacteria (LAB) have been developed as cosmetic ingredients. The topical application of certain LAB strains can improve skin health and combat skin diseases. Here, we investigated the effects of nano-sized lysate of Lactiplantibacillus plantarum APsulloc 331261 (NLAP) isolated from green tea leaves on human skin cells and a reconstructed human epidermis (RHE) model. NLAP increased the expression of genes involved in skin barrier functions such as proliferation, differentiation, tight junction formation, and antimicrobial peptides in normal human epidermal keratinocytes. NLAP prevented the decrease in the expression of differentiation markers and increased release of inflammatory cytokines in keratinocytes cultured with Staphylococcus aureus. NLAP-induced improvements in gene expression and cytokine levels were also observed in RHE treated with heat-killed S. aureus. Additionally, the skin barrier-strengthening effect of NLAP was confirmed by comparing the penetration of the fluorescent dye into the RHE. These findings suggest that NLAP could aid skin barrier function, protect the skin against detrimental bacteria, and suppress inflammatory responses; thus, it can be developed as a skincare ingredient.
Keywords: Lysate · Lantiplantibacillus plantarum APsulloc 331261 · Protection · Skin care

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