Biotechnology and Bioprocess Engineering 2024; 29(2): 353-359  
Efficient production of 1,3‑propanediol from glycerol by a newly isolated soil bacterium using fed‑batch fermentation
Selim Ashoor1,2 · Zhuang Yao1 · Chan Woo Song3 · Haeng Lim Lee1 · Hyeon Jeong Seong1 · Sampathkumar Palaniswamy1 · Jong Myoung Park3 · Hyohak Song3 · Yu‑Sin Jang1
1 Division of Applied Life Science (BK21 Four), Department of Applied Life Chemistry, Institute of Agriculture and Life Science (IALS), Gyeongsang National University (GNU), Jinju 52828, Korea
2 Department of Agricultural Microbiology, Faculty of Agriculture, Ain Shams University, Hadayek Shoubra, Cairo 11241, Egypt
3 R&D Center, GS Caltex Corporation, Daejeon 34122, Korea
Correspondence to: Jong Myoung Park
jmpark@gscaltex.com
Hyohak Song
hyohaks@gscaltex.com
Yu‑Sin Jang
jangys@gnu.ac.kr

Selim Ashoor, Zhuang Yao, Chan Woo Song have equally contributed to this work.
Received: November 6, 2023; Revised: January 24, 2024; Accepted: February 6, 2024; Published online: March 12, 2024.
© The Korean Society for Biotechnology and Bioengineering. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
A total of 817 Lactobacillus-like isolates were obtained from different sources, including soil, ruminant gastric juice, and fermented foods. They were screened to select the best isolate for the production of 1,3-propanediol (1,3-PDO). The isolate SY235 was selected and designated as Lactobacillus reuteri based on the 16S rRNA gene sequence. It was then employed in fed-batch fermentation for producing 1,3-PDO. The highest titer of 1,3-PDO (52.6 ± 2.4 g/L) was attained at 60 h, corresponding to a yield of 0.55 ± 0.01 g/g glycerol and a productivity of 0.88 ± 0.04 g/L/h. No further production was observed after 60 h of incubation. These results demonstrate that the newly isolated strain L. reuteri SY235 can efficiently transform glycerol into 1,3-PDO.
Keywords: Lactobacillus · 1,3-propanediol · Glycerol · Corn steep liquor · Fermentation


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