Biotechnology and Bioprocess Engineering 2024; 29(2): 241-253  
Vibrio species as next‑generation chassis for accelerated synthetic biology
Changhwan Hong1 · Yoojin Kim2 · Hyunjin Lee1,3 · Saebom Yun1 · Hyun Gyu Lim4 · Jina Yang5 · Sungho Jang1,2,3
1 Department of Bioengineering and Nano‑Bioengineering, Incheon National University, Incheon 22012, Korea
2 Division of Bioengineering, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Korea
3 Research Center for Bio Materials and Process Development, Incheon National University, Incheon 22012, Korea
4 Department of Biological Engineering, Inha University, Incheon 22122, Korea
5 Department of Chemical Engineering, Jeju National University, Jeju 63243, Korea
Correspondence to: Jina Yang
Sungho Jang

Changhwan Hong and Yoojin Kim have equally contributed to this work.
Received: November 18, 2023; Revised: November 28, 2023; Accepted: December 3, 2023; Published online: February 19, 2024.
© The Korean Society for Biotechnology and Bioengineering. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Synthetic biology aims to establish engineering principles for biological systems by leveraging the design-build-test-learn (DBTL) cycle. Central to the success of the DBTL cycle is the selection of a suitable chassis, which is the environment in which biological designs are tested. Every step of this cycle is strongly influenced by the properties of chassis. A successful chassis must meet various criteria, prompting ongoing research regarding new candidates. Recently, species within the Vibrio genus, notably Vibrio natriegens and related strains, have emerged as promising next-generation chassis due to their rapid growth rates, versatile substrate utilization, and biosafety level 1 classification. These properties make them highly attractive for accelerating the DBTL cycle with the potential for efficient protein and metabolite production. This review emphasizes the foundational requirements for efficient engineering in synthetic biology, including genetic parts, vectors, and genome engineering technologies tailored to Vibrio species. Practical applications, such as metabolic engineering and protein expression, have been discussed, offering a comprehensive summary of recent advances. This paper also outlines the future directions and suggestions for fully unlocking the potential of Vibrio species as next-generation chassis.
Keywords: Vibrio natriegens · Vibrio species · Chassis strain · Design-build-test-learn cycle · Synthetic biology · Metabolic engineering

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