Biotechnology and Bioprocess Engineering 2024; 29(1): 219-231  
Galactose‑based biohydrogen production from seaweed biomass by novel strain Clostridium sp. JH03 from anaerobic digester sludge
Jeong Hyeon Hwang1 · Hyun Joong Kim1 · Hyun Jin Kim1 · Nara Shin1 · Suk Jin Oh1 · Jeong‑Hoon Park2,3 · Won‑Dong Cho4,5 · Jungoh Ahn4,5 · Shashi Kant Bhatia1 · Yung‑Hun Yang1
1 Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, Korea
2 Clean Energy Transition Group, Korea Institute of Industrial Technology (KITECH), Jeju 63243, Korea
3 Convergence Manufacturing System Engineering, University of Science and Technology (UST), Daejeon 34113, Korea
4 Process Engineering Centre, KRIBB, Cheongju 28116, Korea
5 Bioprocess Department, University of Science and Technology, Daejeon 34113, Korea
Correspondence to: Shashi Kant Bhatia
Yung‑Hun Yang
Received: August 8, 2023; Revised: October 25, 2023; Accepted: November 15, 2023; Published online: February 15, 2024.
© The Korean Society for Biotechnology and Bioengineering. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Seaweed biomass in Korea is rich in galactose following hydrolysis, and leveraging this resource for enhancing the biohydrogen production is the aim of this study. The study investigates the biohydrogen production potential of a newly isolated pure strain, Clostridium sp. JH03, utilizing galactose and seaweed biomass as renewable feedstocks. The strain could utilize galactose as the sole carbon source for biohydrogen production, with a maximum hydrogen yield of 1.61 mol H2/mol galactose. The parameters included pH, temperature, and initial galactose concentration, which were varied to determine the optimal conditions for maximum biohydrogen production. The optimal conditions for biohydrogen production were pH 9 and a temperature of 25 °C, with an initial galactose concentration of 10 g/L. Moreover, hydrogen production from seaweed hydrolysate by Clostridium sp. JH03 resulted in maximum production of 1.71 mol H2/mol galactose. The study also investigated that combining sludge, a common practice in dark fermentation, with JH03 increased biohydrogen production by up to 34%. By addressing the need for clean energy and reducing raw materials price using biomass, this study contributes to the advancement of sustainable and cost-compatible energy solutions.
Keywords: Biohydrogen production · Screening · Galactose · Biomass hydrolysates

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