Biotechnology and Bioprocess Engineering 2024; 29(1): 197-210  
Xylanase enzyme from novel strain and its immobilization onto metal organic framework MOF for fruit juice clarification
Jyoti Kaushal1 · Madhu Khatri1 · Gursharan Singh2 · Shailendra Kumar Arya1
1 Department of Biotechnology, University Institute of Engineering and Technology, Panjab University, Chandigarh 160014, India
2 Department of Medical Laboratory Sciences, Lovely Professional University, Phagwara 144411, Punjab, India
Correspondence to: Shailendra Kumar Arya
skarya_kr@yahoo.co.in; skarya@pu.ac.in
Received: July 6, 2023; Revised: October 9, 2023; Accepted: October 16, 2023; Published online: February 12, 2024.
© The Korean Society for Biotechnology and Bioengineering. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Bacillus pumilus, a bacterial strain was isolated from agricultural soil and used for xylanase enzyme (Xy) production under the submerged fermentation technique. The (Xy) enzyme had an optimum temperature at 50℃ (maximum activity from 45–60 °C) and was active at broad pH range (5.0–8.0) with an optimum pH at around 6.3 as evaluated from response surface methodology studies. This enzyme after purification (purification; 2.87 folds, specific activity; 64.3 U/mg) was immobilized onto MOFCu-BTC (a copper ion-based metal organic framework) and was used for clarification of freshly squeezed fruit juice (pineapple and pomegranate). The study revealed an improved catalytic efficiency (Vmax from 1.252.5 to 1.361 U/mL/mg of support) and greater half-life of the immobilized system (77–99 min). The activation energy decreased from that required for the free system (37.59–25.63 kJ/mol). The reusability of the enzyme improved after immobilization where 61% of the enzyme’s activity was retained after 21 cycles of usage. The MOFXy-Cu-BTC system showed improved clarification (47.58–57.97% for pineapple, and 15.34–18.3 for pomegranate) thereby showing its effectiveness in commercial juice clarification process.
Keywords: Bacillus pumilus · Thermo tolerant · Xylanase · Metal organic framework · Juice clarification


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