Biotechnology and Bioprocess Engineering 2023; 28(6): 949-961  
Quantitative Methods for Metabolite Analysis in Metabolic Engineering
Cheeyoon Ahn, Min-Kyung Lee, and Cheulhee Jung
Cheeyoon Ahn, Min-Kyung Lee, Cheulhee Jung*
Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Korea
Tel: +82-2-3290-3023
E-mail: damo363@korea.ac.kr
Received: June 30, 2022; Revised: September 18, 2022; Accepted: October 2, 2022; Published online: December 31, 2023.
© The Korean Society for Biotechnology and Bioengineering. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
We present a brief overview of metabolic engineering, depicting the necessity of exploiting microorganisms for obtaining desired metabolites and the difficulty of metabolic pathway optimization under numerous conditions. The advantages, limitations, and examples of conventional quantitative analytical methods that focus on accuracy but have low throughput rates are presented. We have also described in vivo analytical methods with high-throughput rates, which indirectly compare the yield of the reporter protein. Additionally, we have explained a few considerations for engineering in vivo analytical methods. This review also highlights the current challenges faced by the analytical methods in the metabolic engineering.
Keywords: metabolic engineering, metabolite analysis, throughput, metabolite-responsive transcription factor, riboswitch


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