Biotechnology and Bioprocess Engineering 2023; 28(3): 379-385  
Impact of the Synthetic Scaffold Strategy on the Metabolic Pathway Engineering
Kim-Ngan T Tran, Ashokkumar Kumaravel, and Soon Ho Hong
Kim-Ngan T Tran, Ashokkumar Kumaravel, Soon Ho Hong*
Department of Chemical Engineering, University of Ulsan, Ulsan 44610, Korea
Tel: +82-52-259-1293; Fax: +82-52-259-1689
Kim-Ngan T Tran and Ashokkumar Kumaravel are co-first authors and contributed equally to this work.
Received: November 14, 2022; Revised: March 7, 2023; Accepted: March 13, 2023; Published online: June 30, 2023.
© The Korean Society for Biotechnology and Bioengineering. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
For the development of the efficient bio-refinery process or biochemical producer, metabolic engineering has become an attractive choice recently. However, engineered metabolic pathways often suffer from flux imbalances due to a lack of corresponding regulatory mechanisms associated with natural metabolism. The interaction among different enzymes within a metabolic pathway plays an important role in regulating the efficiency of metabolic processes. Consequently, the creation of protein scaffolds has helped with the spatial co-localization of proteins in metabolic engineering. Research on protein scaffolds indicated scaffold systems may enhance metabolic productivity further. In this review, the specificity, selectivity, and regulatory mechanisms of protein-protein interactions are discussed in the context of the important effects that they exert on various biological processes.
Keywords: scaffold protein, metabolic engineering, synthetic biology, Escherichia coli

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