Biotechnology and Bioprocess Engineering 2022; 27(3): 415-422  
Improved Extracellular Enzyme-mediated Production of 7,10-dihydroxy-8(E)-octadecenoic Acid by Pseudomonas aeruginosa
Ji Wan Jeong, Mamata Singhvi, and Beom Soo Kim
Ji Wan Jeong, Mamata Singhvi, Beom Soo Kim*
Department of Chemical Engineering, Chungbuk National University, Cheongju 28644, Korea
Tel: +82-43-261-2372; Fax: +82-43-269-2370
E-mail: bskim@chungbuk.ac.kr
Received: August 20, 2021; Revised: October 20, 2021; Accepted: November 4, 2021; Published online: June 30, 2022.
© The Korean Society for Biotechnology and Bioengineering. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
In this study, 7,10-dihydroxy-8(E)-octadecenoic acid (DOD) was produced using extracellular enzymes present in the supernatant of Pseudomonas aeruginosa. DOD concentration and productivity were improved using extracellular enzymes obtained from P. aeruginosa culture broth than using conventional whole cell culture method. Among the different carbon sources used during cell preculture, glycerol showed higher DOD production than using glucose or crude glycerol. In batch process, the highest concentration of DOD (8.82 g/L) was accomplished using supernatant derived from 12 h-grown pre-culture of P. aeruginosa strain after 72 h of bioconversion process. To further improve DOD production, a fed-batch process was used with the addition of surfactant and enzyme concentrate. The fed-batch process using a 4-fold concentrated enzyme solution with Tween 80 yielded the highest DOD concentration (27.5 g/L at 72 h) with an 8.28-fold increase in DOD production compared to using whole cells. The prominence of this study was that the highest DOD production (27.5 g/L) ever reported was achieved using extracellular enzymes via a fed-batch process. Another significant aspect of this study is the possibility of utilizing abundantly available crude glycerol substrate for DOD production, which will help direct the development of sustainable process. Therefore, the overall study is projected to speed up the development of DOD production using inexpensive substrates such as crude glycerol on a larger scale.
Keywords: 7,10-dihydroxy-8(E)-octadecenoic acid, Pseudomonas aeruginosa, extracellular enzyme, oleic acid, fedbatch process


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