Biotechnology and Bioprocess Engineering 2022; 27(3): 379-385  
Theabrownin in Black Tea Suppresses UVB-induced Matrix Metalloproteinase-1 Expression in HaCaT Keratinocytes
Hyung-Min Kim, Eun-Mi Kim, Eun-Soo Lee, Nok Hyun Park, Yong Deog Hong, and Ji-Yong Jung
Hyung-Min Kim, Eun-Mi Kim, Eun-Soo Lee, Nok Hyun Park, Yong Deog Hong, Ji-Yong Jung*
Basic Research and Innovation Division, R&D Center, Amorepacific Corporation, Yongin 17074, Korea
Tel: +82-31-899-2691; Fax: +82-31-899-2594
Hyung-Min Kim
Department of Chemistry and Cosmetics, Jeju National University, Jeju 63243, Korea
Received: November 1, 2021; Revised: December 19, 2021; Accepted: December 22, 2021; Published online: June 30, 2022.
© The Korean Society for Biotechnology and Bioengineering. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Theabrownin is a heterogeneous polyphenolic compound, obtained from fermented dark teas, with many bioactive functions. However, the role of theabrownin in skin photoaging remains unclear. In this study, we investigated whether theabrownin modulates ultraviolet B (UVB)-induced matrix metalloprotease-1 (MMP-1) expression in HaCaT keratinocytes. Theabrownin exerted antioxidant properties, which resulted in scavenging of intracellular reactive oxygen species generated by UVB irradiation in HaCaT cells. Consequently, theabrownin inhibited UVB-induced extracellular signal-regulated kinase, p38, and c-Jun N-terminal kinase phosphorylation, which are activated by oxidative stress. Theabrownin also suppressed the downstream signaling pathway of mitogen-activated protein kinase and the nuclear accumulation of activator protein-1, which is an essential transcription factor for UVB-stimulated MMP-1 expression. Based on these results, we conclude that theabrownin can serve as a novel antiphotoaging compound for the skin.
Keywords: theabrownin, photoaging, antioxidant, UVB irradiation, matrix metalloproteinase-1, keratinocytes

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