Biotechnology and Bioprocess Engineering 2022; 27(2): 286-293  
Selective Recovery of L-pipecolic Acid from L-lysine Bioconversion Mixture by Liquid-liquid Extraction
Sun Mi Lee, Hong-Ju Lee, Sang Hyun Kim, Min Ju Suh, Jang Yeon Cho, Sion Ham, Ranjit Gurav, Sang Hyun Lee, Shashi Kant Bhatia, and Yung-Hun Yang
Sun Mi Lee, Hong-Ju Lee, Sang Hyun Kim, Min Ju Suh, Jang Yeon Cho, Sion Ham, Ranjit Gurav, Sang Hyun Lee, Shashi Kant Bhatia*, Yung-Hun Yang*
Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, Korea
Tel: +82-2-450-3936
E-mail: shashibiotechhpu@gmail.com
E-mail: seokor@konkuk.ac.kr
Received: August 22, 2021; Revised: October 13, 2021; Accepted: October 13, 2021; Published online: April 30, 2022.
© The Korean Society for Biotechnology and Bioengineering. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
L-pipecolic acid (L-PA) is a key chiral intermediate in the synthesis of many important drugs. However, there is a lack of research on the efficient extraction and purification process for L-PA. Here, eleven different organic solvents were evaluated for selective extraction of L-PA by physical extraction. Among these solvents, methyl ethyl ketone (MEK) showed the highest extraction yield of LPA, and analysis of the linear solvation energy relationship between the solvents showed that MEK was the most suitable solvent for L-PA extraction. The highest extraction yield of L-PA was 37% under optimal conditions (pH 6, 20°C, 30 min and ratio of 1). Furthermore, the repetitive extraction of L-PA from bioconversion solution was also investigated. The MEK gave high purity of L-PA (93.1% and 22.8% yield) from the bioconversion mixture. Our study is the first approach to show easy recovery of L-PA by liquid-liquid extraction and the application of MEK extraction method to L-lysine bioconversion mixture.
Keywords: pipecolic acid, physical extraction, methyl ethyl ketone, linear solvation energy relationship


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