Biotechnology and Bioprocess Engineering 2021; 26(6): 1012-1022  
Optimization and Modeling of Curdlan Production under Multi-physiological-parameters Process Control by Agrobacterium radiobacter Mutant A-15 at High Initial Glucose
Hui Yang, Wenqiang Li, Shuochang Chen, Xiaolei Guo, Fei Huang, and Ping Zhu
Hui Yang*, Wenqiang Li, Shuochang Chen, Xiaolei Guo, Fei Huang, Ping Zhu
College of Life Science and Technology, Guangxi University, Nanning, Guangxi 530004, China
Tel: +86-0771-3235707
Hui Yang
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning, Guangxi 530004, China
Guangxi Research Center for Microbial and Enzyme Engineering Technology, Guangxi University, Nanning, Guangxi 530004, China
These authors contributed equally to this work.
Received: January 31, 2021; Revised: April 23, 2021; Accepted: April 25, 2021; Published online: December 31, 2021.
© The Korean Society for Biotechnology and Bioengineering. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
The optimization and kinetic modeling of curdlan synthesis by Agrobacterium radiobacter mutant A-15 under multi-physiological-parameters process control, especially employing the on-line oxygen uptake rate (OUR) and carbon dioxide evolution rate (CER) were performed in a 50-L fermentor. Results indicated that the maximum production of curdlan reach about 32.5 g/L with a glucose conversion yield of 49.5%, the maximum specific growth rate was about 0.5338 h-1, the kinetics equation were of positive significance for production. It also demonstrated that A-15 was insensitive to catabolite repression during polysaccharide production for its maintaining high conversion yield in the medium contained 6.6% (W/V) initial glucose. In addition, the OUR and CER at the stationary growth stage showed a positive correlation with curdlan synthesis. This work will provide more theoretical support for large-scale curdlan production.
Keywords: curdlan, optimization, OUR, CER, kinetic model

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