Biotechnology and Bioprocess Engineering 2021; 26(6): 910-922  
Characterization and Functional Analysis of Bacillus aryabhattai CY for Acrylic Acid Biodegradation: Immobilization and Metabolic Pathway
Yi Chen, Min Zhao, Liyong Hu, Zeyu Wang, Dzmitry Hrynsphan, and Jun Chen
Yi Chen, Min Zhao, Liyong Hu
College of Environment, Zhejiang University of Technology, Hangzhou 310032, China
Zeyu Wang, Jun Chen*
Interdisciplinary Research Academy, Zhejiang Shuren University, Hangzhou 310021, China
Tel:+86-571-88285209; Fax: +86-571-88285209
E-mail: bec@zjut.edu.cn
Dzmitry Hrynsphan
Research Institute of Physical and Chemical Problems, Blearusian State University, Minsk 220030, Belarus
Received: February 7, 2021; Revised: April 9, 2021; Accepted: April 23, 2021; Published online: December 31, 2021.
© The Korean Society for Biotechnology and Bioengineering. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Acrylic acid has been widely used in various industrial applications but is harmful to human health and the environment. A novel and efficient degrading acrylic acid bacterium was isolated and identified as Bacillus aryabhattai CY. In this study, batch experiments were conducted to evaluate the biodegradation of acrylic acid by B. aryabhattai CY, which were immobilized in calciumalginate beads under different conditions. The components of the alginate beads were optimized by the response surface method, and the degradation performance of the immobilized cells was determined. Relative to the free cells, experiment results showed that the immobilized cells can achieve complete degradation of 100 mg/L acrylic acid in 24 h under the optimal conditions of SA 6% (w/v), CaCl2 1% (w/v), and immobilization time of 6 h. According to Haldane's model, the maximum specific growth rate (μmax) of the free cells and immobilized cells were 0.165/h and 0.210/h, respectively. Experiment data revealed that acrylic acid showed an inhibitory effect on biodegradation by B. aryabhattai CY, especially at concentration higher than 100 mg/L. Furthermore, the reusability of the immobilized cells revealed that the acrylic acid removal rate was above 93.70% within the eight cycles. The immobilized cells also showed higher removal efficiencies in wider ranges of temperature (20°C-60°C) and pH (5.0-10.0) than the free cells. Moreover, the possible degradation intermediates were proposed during the biodegradation of acrylic acid by GC-MS analysis. Results indicated that immobilized beads might have a potential environmental implication in the purification of practical acrylic acid wastewater.
Keywords: acrylic acid, biodegradation, immobilization, Bacillus aryabhattai CY, Haldane's model


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