Biotechnology and Bioprocess Engineering 2021; 26(4): 660-668  
Kinetic and Thermodynamic Study of the Ultrasonic Acetone-pentane Fractional Precipitation of Paclitaxel from the Plant Cell Cultures of Taxus chinensis
Hye-Su Min and Jin-Hyun Kim
Hye-Su Min, Jin-Hyun Kim*
Department of Chemical Engineering, Kongju National University, Cheonan 31080, Korea
Tel: +82-41-521-9361; Fax: +82-41-554-2640
Received: October 29, 2020; Revised: December 4, 2020; Accepted: December 4, 2020; Published online: August 30, 2021.
© The Korean Society for Biotechnology and Bioengineering. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
In this study, the purification efficiency of paclitaxel was remarkably improved through ultrasonic acetone-pentane fractional precipitation. The time taken for precipitation was also shortened by a maximum of 192-fold (at 5°C, 180-250 W) with ultrasound as compared to that of the conventional method. The precipitation was completed within 30 min regardless of temperature (5-25°C), thereby improving the operating conditions (precipitation time and temperature) as compared to the conventional method. In addition, the rate constant in the fractional precipitation with ultrasound (80-250 W) was also increased by 8-to 13-fold (at 5°C) as compared to the conventional method. The standard enthalpy change and standard entropy change were negative, while the standard Gibbs free energy change was positive. These results indicate that the precipitation process was exothermic, irreversible, and non-spontaneous.
Keywords: paclitaxel, acetone-pentane fractional precipitation, ultrasound, kinetics, thermodynamics

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