Biotechnology and Bioprocess Engineering 2020; 25(6): 862-871  
Current Status of Pseudomonas putida Engineering for Lignin Valorization
Siseon Lee, Jung-Hoon Sohn, Jung-Hoon Bae, Sun Chang Kim, and Bong Hyun Sung
Siseon Lee, Jung-Hoon Sohn, Jung-Hoon Bae, Bong Hyun Sung*
Synthetic Biology and Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Korea
Tel:+82-42-860-4472; Fax:+82-42-860-4489
E-mail: bhsung@kribb.re.kr
Siseon Lee, Sun Chang Kim
Intelligent Synthetic Biology Center, Daejeon 34141, Korea
Jung-Hoon Sohn, Bong Hyun Sung
Department of Biosystems and Bioengineering, Korea University of Science and Technology (UST), Daejeon 34113, Korea
Received: January 27, 2020; Revised: April 24, 2020; Accepted: May 4, 2020; Published online: December 31, 2020.
© The Korean Society for Biotechnology and Bioengineering. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Lignin, a complex aromatic polymer, is a structural component of plant biomass. It decomposes with difficulty because of its rigidity properties, however, lignin valorization is essential for the economics of lignocellulosic biorefineries. Pseudomonas putida has been extensively investigated as a promising host strain for lignin valorizations due to intrinsic traits, such as low nutritional requirement, high tolerance to toxicity, and metabolic versatility with a wide spectrum of substrates, such as aromatic compounds. Although a naturally occurring, lignin-utilizing P. putida strain has been reported, it is necessary to engineer the genome of P. putida for efficient lignin utilization. For biological lignin valorization, the decomposition of lignin polymer to low-molecular weight compounds and transformation of lignin-derived aromatic compounds to valueadded chemicals is essential. Various tools of synthetic biology have been developed for the genome engineering of P. putida; efforts in metabolic engineering have been made to expand aromatic substrate specificity and to improve productivity of value-added chemicals. Development of high-performance bio-parts and biosensors for lignin valorization has also been done. In this review, we present recent research on genome engineering tools developed for P. putida and metabolic engineering employed in P. putida to improve lignin valorization.
Keywords: Pseudomonas putida, lignin valorization, synthetic biology, biosensor

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