Biotechnology and Bioprocess Engineering 2020; 25(1): 39-44  
Enhanced Production of Malic Acid by Co-localization of Phosphoenolpyruvate Carboxylase and Malate Dehydrogenase Using Synthetic Protein Scaffold in Escherichia coli
Sivachandiran Somasundaram1, Jaehoon Jeong1, Ganesh Irisappan1, Tae Wan Kim2,*, and Soon Ho Hong1,*
1Department of Chemical Engineering, University of Ulsan, Ulsan 44610, Korea
2Department of Biotechnology & Bioengineering, Chonnam National University, Gwangju 61186, Korea
Correspondence to: Soon Ho Hong*
Department of Chemical Engineering, University of Ulsan, Ulsan 44610, Korea
Tel: +82-52-259-1293; Fax: +82-52-259-2247
E-mail: shhong@ulsan.ac.kr
Tae Wan Kim*
Department of Biotechnology & Bioengineering, Chonnam National University, Gwangju 61186, Korea
Tel: +82-62-530-1842; Fax: +82-62-530-1949
E-mail: chekimtw@chonnam.ac.kr
Received: July 9, 2019; Revised: October 11, 2019; Accepted: October 12, 2019; Published online: February 29, 2020.
© The Korean Society for Biotechnology and Bioengineering. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
To produce malic acid from non-oxidative pathway route in Escherichia coli using two key enzymes and synthetic scaffold complex. E. coli was engineered to produce malic acid from glucose by co-localization of two key enzymes phosphoenolpyruvate carboxylase (Ppc) and malate dehydrogenase (MdhA) with synthetic scaffold complex. Scaffold plasmid has produced the maximum concentration of 3.51 g/L malic acid from 10 g/L glucose in 48 h of culture. pH 5.5 and temperature 30°C were optimum for malic acid production without any engineering of competing metabolic pathways. E. coli mutant strains and different concentrations of glucose also tested. When 50 g/L glucose was used as substrate, 20.4 g/L of malic acid was produced.
Keywords: co-localization, Escherichia coli, malic acid, protein scaffold


This Article

Archives