Biotechnology and Bioprocess Engineering 2020; 25(1): 29-38  
Roles of Macrophage Colony Stimulating Factor in White and Brown Adipocytes
Sulagna Mukherjee1,†, Kanikkai Raja Aseer1,†, and Jong Won Yun1,*
Department of Biotechnology, Daegu University, Gyeongsan 38453, Korea
Correspondence to: Jong Won Yun*
Department of Biotechnology, Daegu University, Gyeongsan 38453, Korea
Tel: +82-53-850-6556; Fax: +82-53-850-6559

These two authors contributed equally to this study.
Received: January 19, 2020; Revised: January 26, 2020; Accepted: January 30, 2020; Published online: February 29, 2020.
© The Korean Society for Biotechnology and Bioengineering. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Macrophage colony stimulating factor (M-CSF), commonly known as CSF1, is a cytokine produced by osteoblast cells, and is well known for its important role in differentiation of osteoclasts and white adipocyte hyperplasia. However, the role of M-CSF in lipid metabolic activity is lacking insight. In the current study, we explore unidentified actions of M-CSF in adipocytes. Levels of M-CSF were determined for white and brown adipose tissues and cells, and the expression levels of the important protein markers in lipid metabolism were evaluated through immunoblot analysis, subsequent to blocking M-CSF in fat cells by silencing its receptor Csf1r. Interestingly, we observed presence of M-CSF in brown fat cells. In addition, the lack of M-CSF in HIB1B cells attenuated the expressions of protein markers responsible for lipid metabolism in the brown adipocytes. Further, deficiency of M-CSF resulted in decreased adipogenesis along with reduced expression of lipogenic markers for both white and brown adipocytes. Moreover, lipid metabolism was balanced by M-CSF via the PPARγ-mediated LPL activity in white adipocytes. Taken together, the results from this study indicate that M-CSF is an important regulatory protein in both white and brown adipocytes.
Keywords: adipocytes, anti-obesity, M-CSF, PPAR gamma

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