Biotechnology and Bioprocess Engineering 2020; 25(1): 22-28  
Inhibition of 3T3-L1 Adipocyte Differentiation by D-allulose
Seohyun Moon1, Yang Hee Kim2, and Kyungoh Choi1,*
1Department of Chemical and Material Engineering, The University of Suwon, Hwaseong 18323, Korea
2Food Research Institute, CJ CheilJedang, Suwon 16495, Korea
Correspondence to: Kyungoh Choi*
Department of Chemical and Material Engineering, The University of Suwon, Hwaseong 18323, Korea
Tel: +82-31-229-8542; Fax: +82-31-220-2528
E-mail: kyungoh98@suwon.ac.kr
Received: September 7, 2019; Revised: October 13, 2019; Accepted: October 16, 2019; Published online: February 29, 2020.
© The Korean Society for Biotechnology and Bioengineering. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Obesity is a serious problem in modern society and its prevalence continues to increase worldwide, resulting in metabolic disorder related diseases. D-allulose, a sugar substitute, boasts a near-zero calorie value and regulates lipid accumulation. However, the molecular mechanism of D-allulose at the cellular level has not been fully elucidated. In this study, we investigated the effect of D-allulose on 3T3-L1 adipocyte differentiation. D-allulose inhibits differentiation of 3T3-L1 preadipocytes into mature adipocytes, as examined by Oil Red O staining. The mRNA levels of genes involved in lipogenesis, including fatty acid synthase (FAS) and adipocyte fatty acid-binding protein (aP2), were significantly decreased and intracellular triglyceride (TG) content was markedly reduced with D-allulose treatment. We also monitored the activity of major adipogenic transcription factors – CREB, SREBP-1c, and PPARγ – using 3T3-L1 reporter cell lines that were constructed to secrete Gaussia luciferase upon binding of a transcription factor to its DNA binding element. Collectively, D-allulose suppresses adipocyte differentiation and lipid accumulation through regulating adipogenic transcription factors and may have anti-obesity potential.
Keywords: D-allulose, adipocyte differentiation, obesity, lipid


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