Biotechnology and Bioprocess Engineering 2019; 24(6): 876-884  
Immobilized Laminin-derived Peptide Can Enhance Expression of Stemness Markers in Mesenchymal Stem Cells
Nafiseh Tavakolpoor Saleh1, Alireza Naderi Sohi2,*, Elaheh Esmaeili2,3, Somayeh Karami2,4, Fatemeh Soleimanifar5, and Nikoo Nasoohi1
1Department of Biochemistry, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran 1916893813, Iran
2Stem Cell Technology Research Center, Tehran 1997775555, Iran
3Hematology and Cell Therapy Department, School of Medical Sciences, Tarbiat Modares University, Tehran 14115-111, Iran
4Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran 1916893813, Iran
5Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran
Correspondence to: Alireza Naderi Sohi*
Stem Cell Technology Research Center, Tehran 1997775555, Iran
Tel: +98-2122082120; Fax: +98-2122351460
E-mail: nanosohi@gmail.com
Received: April 1, 2019; Revised: July 21, 2019; Accepted: July 23, 2019; Published online: December 31, 2019.
© The Korean Society for Biotechnology and Bioengineering. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Due to number of reasons such as ease of isolation and their broad differentiation capability, adult human mesenchymal stem cells (MSCs) are widely considered as of the most promising cells for regenerative medicine and tissue engineering. Nevertheless quick decrease in expression of transcription factors associated with stemness and self-renewal during ex vivo expansion of MSCs is an impediment against their therapeutic applications. Since the influence of extra cellular matrix (ECM) proteins on the fate of stem cells is well documented, the culture of MSCs on ECM-derived synthetic biomolecules is worth investigating. In the present study, a laminin-derived peptide, YIGSR was covalently immobilized on the chitosan film surface using carbodiimide chemistry and confirmed by fluorometry. The results obtained from surface characterization by atomic force microscopy (AFM) and contact angle measurement, showed no significant difference in topological features and hydrophilicity after peptide immobilization. Employment of these surfaces for culture of human adipose-derived mesenchymal stem cells demonstrated that the immobilized YIGSR peptide has a favorable effect on adhesion and maintaining viability of the MSCs as well as on the expression of stemness markers (Nanog, Oct-4, and Sox-2) in these cells.
Keywords: chitosan, immobilization, mesenchymal stem cells, stemness markers, YIGSR peptide


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