Biotechnology and Bioprocess Engineering 2019; 24(5): 818-823  
Effective Diffusivity and Mass Transfer Coefficient during the Extraction of Paclitaxel from Taxus chinensis Using Methanol
Ye-Ji Jo and Jin-Hyun Kim*
Department of Chemical Engineering, Kongju National University, Cheonan 31080, Korea
Correspondence to: Jin-Hyun Kim
Department of Chemical Engineering, Kongju National University, Cheonan 31080, Korea
Tel: +82-41-521-9361; Fax: +82-41-554-2640
E-mail: jinhyun@kongju.ac.kr
Received: April 19, 2019; Revised: May 28, 2019; Accepted: June 23, 2019; Published online: October 31, 2019.
© The Korean Society for Biotechnology and Bioengineering. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Solid-liquid extraction of paclitaxel from the biomass of Taxus chinensis was investigated in order to understand the effect of temperature, solvent concentration and stirring speed on the mechanism and kinetics of extraction. Paclitaxel concentration-time data were analyzed using a second-order kinetic model to determine the kinetic parameters. A diffusion model was utilized to determine diffusivity and mass transfer coefficient taking into account both washing and diffusion phases together. The effective diffusion coefficient (1.2814 × 10-13-5.9765 × 10-13 m2/s), mass transfer coefficient (1.0020 × 10-7-1.2598 × 10-7 m/s), and Biot number (3.927-8.959) increased with increasing temperature (298-318 K), methanol concentration (75-100%) and stirring speed (170-570 rpm), which indicated that the external resistance to mass transfer is negligible due to efficient mixing of solute and solvent.
Keywords: paclitaxel, solid-liquid extraction, kinetics, effective diffusivity, mass transfer coefficient


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