Biotechnology and Bioprocess Engineering 2019; 24(5): 754-760  
Comparison of Cationic Flocculants for the Clarification of CHO-derived Monoclonal Antibodies
Rimenys Junior Carvalho*
Cell Culture Engineering Laboratory (LECC), Chemical Engineering Program, COPPE, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro 21941-598, Brazil
Correspondence to: Rimenys Junior Carvalho
Cell Culture Engineering Laboratory (LECC), Chemical Engineering Program, COPPE, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro 21941-598, Brazil
Tel: +55-21-3938-7163; Fax: +55 -21-3938-8300
E-mail: rjc@peq.coppe.ufrj.br
Received: April 25, 2019; Revised: July 9, 2019; Accepted: July 9, 2019; Published online: October 31, 2019.
© The Korean Society for Biotechnology and Bioengineering. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
This work used two different cationic polyelectrolytes as flocculation agents for the clarification of growth medium from Chinese hamster ovary (CHO) monoclonal antibodies (mAb)-producing cells, namely chitosan and poly diallyldimethylammonium chloride (pDADMAC). The conditions set for the use of pDADMAC consisted in the variation of the polymer's concentrations, in light of largely available literature. Chitosan was applied to a broader concentration-range under two different pH values, for its use in CHO cells flocculation is unprecedented in the literature. Chitosan presented the best performance on clarification assays under a concentration 9-fold lower compared with pDADMAC (5 pg/total cell (TC) and 45 pg/TC, respectively), albeit both agents provided similar results regarding sedimentation rates (15min), DNA removal (98%), and mAb recovery (90%). Despite the expected low proteins removal, namely 11.1% in the case of chitosan and no removal for pDADMAC, the flocculation technique proved to be cost-effective. Hence, its application can be envisioned not only when the clarification of a growth medium from CHO cells production is considered, but also for the removal of soluble impurities in a single step.
Keywords: Chinese hamster ovary cells, clarification, flocculation, monoclonal antibodies, chitosan, pDADMAC


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