Biotechnology and Bioprocess Engineering 2019; 24(3): 476-482  
AHCC Supplementation Attenuates Muscle Atrophy via Akt Activation in Hindlimb-suspended Rat
Young-Ju Song1, Sang-Hoon Bae2, Jin-Young Park2, Hye-Jin Lee3, My-Young Lee3, Seung-Jea Lee4, Young-Ki Min5, and Jeong-Beom Lee5,*
1Institute of Sports Health Science, Sunmoon University, Asan 31460, Korea
2Department of physical education, Graduate School of Sunmoon University, Asan 31460, Korea
3Global Graduate School of Healthcare Business, Soonchunhyang University, Asan 31538, Korea
4Department of Medical Science, Soonchunhyang University, Asan 31538, Korea
5Department of Physiology, College of Medicine, Soonchunhyang University, Cheonan 31151, Korea
Correspondence to: Jeong-Beom Lee
Tel: +82-10-2423-5317, +82-41-570-2436; Fax: +82-41-570-2430
E-mail: leejb@sch.ac.kr
Received: December 16, 2018; Revised: April 15, 2019; Accepted: April 16, 2019; Published online: June 30, 2019.
© The Korean Society for Biotechnology and Bioengineering. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
We investigated the inhibitory effect of active hexose correlated compound (AHCC) on muscle atrophy in hindlimb-suspended rats. Twenty-four six-week-old male Sprague-Dawley rats were randomly divided into three groups: the control sedentary group (CS, n = 8), the hindlimb-suspended group (HS, n = 8), and the hindlimb-suspended and AHCC-supplemented group (HSA, n = 8). Hindlimb suspension and AHCC supplementation were performed for two weeks. The HSA group was treated with AHCC (1 g/1 kg of body weight (BW)) orally in 0.3 mL of PBS solution, while the HS group received the vehicle (PBS solution) only. After two weeks, the cross-sectional area (CSA) of the HS and HSA groups decreased by approximately 36% (p < 0.05) and 19%, respectively, compared to the CS group. In addition, myonuclear numbers of the HS and HSA groups and the extensor digitorum longus (EDL) muscle weight of the HS group decreased 30% (p < 0.05) and 18%, respectively, compared to the CS group. AHCC supplementation increased the phosphorylation of pAkt/Akt in the HSA group compared to the HS group (p < 0.05). Furthermore, Fbx32 and MuRF1 protein expression in the HSA group recovered to the level of the CS group. Based on these results, AHCC supplementation may have a positive role in the prevention of muscle atrophy via Akt activation in hindlimb-suspended rats.
Keywords: AHCC, hindlimb suspension, myonuclei, CSA, muscle atrophy


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